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Human Monoclonal Antibodies to Rh o (D)
Author(s) -
Foung Steven K.H.,
Blunt Judith A.,
Wu Paul S.,
Ahearn Patricia,
Winn Linda C.,
Engleman Edgar G.,
Grumet F. Carl
Publication year - 1987
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1987.tb04912.x
Subject(s) - monoclonal antibody , antibody , epitope , hemagglutination , microbiology and biotechnology , antigen , hemagglutination assay , rh blood group system , virology , chemistry , immunoglobulin m , monoclonal , biology , immunology , immunoglobulin g , titer
. B lymphocytes from Rh negative donors with serum anti‐D antibodies were isolated and fused with the mouse‐human heteromyeloma, SBC‐H20, to produce hybridomas secreting IgM or IgG 1 human monoclonal antibodies to D antigen. The IgM antibody in hybridoma supernatant agglutinates all normal D positive cells at the immediate spin phase of reactivity. Using concentrated IgM hybridoma supernatant of approximately 50 μg/ml, D u cells were also agglutinated. The IgG 1 antibody reacts by indirect hemagglutination with all D and D u cells. Against Rh mosaics, different reactivity was noted for each antibody. Furthermore, D positive cells precoated with the IgG 1 antibody inhibit the IgM direct hemagglutination, suggesting that the antibodies identify closely associated epitopes. These human monoclonal antibodies will be useful diagnostic reagents and, ultimately, should be useful in the prevention of Rh hemolytic disease of the newborn.