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Effects of Partial and Total Isovolemic Exchange Transfusion in Fully Conscious Rats Using Pyridoxylated Polyhemoglobin Solution as a Colloidal Oxygen‐Delivering Blood Replacement Fluid 1
Author(s) -
Keipert P. E.,
Chang T. M. S.
Publication year - 1987
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1987.tb04905.x
Subject(s) - oncotic pressure , exchange transfusion , albumin , chemistry , hemoglobin , blood transfusion , blood pressure , fluid replacement , hemodynamics , colloid , resuscitation , oxygen , anesthesia , medicine , biochemistry , organic chemistry
. Stroma‐free hemoglobin (SFHb) treated with pyridoxal 5′‐phosphate (PP) was intermolecularly cross‐linked with glutaraldehyde to give pyridoxylated polyhemoglobin (PP‐PolyHb) solution (14.0 g/dl) with colloid osmotic pressure = 25 torr and a P 50 = 20 torr. To evaluate PP‐PolyHb as a blood substitute, fully conscious rats with chronic cannulations were subjected to blood replacement (30–100%) by a new continuous isovolemic exchange transfusion set up. Crystalline SFHb, PP‐SFHb, PP‐PolyHb, bovine serum albumin (BSA) and Ringer's solution were tested. Respiration, pulse and arterial blood pressure were more stable following transfusion with PP‐PolyHb or PP‐SFHb compared to BSA. By 7–8 days post‐exchange, hematological changes returned to normal. Plasma retention of PP‐PolyHb was 10–15 times longer than either crystalline SFHb or PP‐SFHb for major blood exchanges. A T 1/2 of 30 h was obtained for PP‐PolyHb after total replacement. Long‐term survival rates of 80, 63 and 50%, following replacement of 70, 85 and 100%, respectively, were significantly higher (p<0.05) than survival obtained for all other solutions tested. Superiority of PP‐PolyHb over other fluids was clearly demonstrated.