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Severe Hemolytic Disease of the Newborn due to Anti‐Vw and Detection of Glycophorin A Antigens on the Miltenberger I Sialoglycoprotein by Western Blotting
Author(s) -
Rearden A.,
Frandson S.,
Carry J.B.
Publication year - 1987
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1987.tb04900.x
Subject(s) - glycophorin , sialoglycoprotein , antigen , blot , microbiology and biotechnology , trypsin , antibody , gel electrophoresis , monoclonal antibody , red blood cell , staining , polyacrylamide gel electrophoresis , biology , western blot , chemistry , immunology , biochemistry , enzyme , genetics , gene
. Anti‐Vw detecting an antigen on Miltenberger I (Mi I) variant glycophorin A (GPA) has rarely been reported as a cause of hemolytic disease of the newborn (HDN). We report an infant with severe HDN due to anti‐Vw. Examination of the Vw+ erythrocytes of the father and paternal grandmother by sodium dodecylsulphate polyacrylamide gel electrophoresis showed an extra trypsin‐sensitive, periodic‐acid‐Schiff staining band, consistent with Mi I variant GPA. Staining of Western blots by monoclonal antibodies showed that normal paternal GPA expressed blood group M, while Mi I variant GPA expressed blood group N. Mi I variant GPA expressed the trypsin‐sensitive antigenic determinant detected by MoAb 10F7, indicating that the alterations known to occur in the trypsin‐sensitive fragment of Mi I variant GPA do not affect expression of the antigen detected by 10F7.