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Human Erythrocyte Antigens
Author(s) -
Telen Marilyn J.,
Scearce Richard M.,
Haynes Barton F.
Publication year - 1987
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1987.tb03035.x
Subject(s) - sialoglycoprotein , glycophorin , antibody , monoclonal antibody , antigen , band 3 , microbiology and biotechnology , biology , red blood cell , rh blood group system , membrane protein , chemistry , immunology , biochemistry , membrane
. Human erythrocyte membrane proteins express antigens which serve as markers for erythroid differentiation as well as targets for human blood group alloantibodies. We have produced and characterized a new panel of five monoclonal antibodies to erythrocyte membrane proteins. Three monoclonal antibodies (E3, E4, E5) were specific for erythrocyte glycophorins. One antibody (E3) identified the sialoglycoprotein α and β homologous regions proximal to the plasma membrane, a second antibody (E4) was specific for sialoglycoprotein α, while a third (E5) was a sialogylcoprotein‐β‐specific antibody. Two antibodies (E6 and TE10) to the 65,000‐dalton chymotrypsin cleavage product of band 3 were also produced. These antibodies constitute a new panel of probes for investigation of normal erythroid differentiation, erythroleukemia, and the expression of normal and anomalous blood group antigens.