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Murine Monoclonal Antibodies to Human A Erythrocytes: Differential Reactivity with N‐acetyl‐ D ‐galactosamine
Author(s) -
Němec M.,
Dřimalová D.,
Hořejši V.,
Vaňák J.,
Bártek J.,
Viklický V.
Publication year - 1987
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1987.tb03007.x
Subject(s) - monoclonal antibody , agglutination (biology) , microbiology and biotechnology , chemistry , galactosamine , antibody , dissociation constant , red blood cell , antigen , epitope , hybridoma technology , biology , biochemistry , immunology , receptor , glucosamine
. Six different hybridoma cell lines were obtained producing IgM monoclonal antibodies (MAbs) against human blood group A antigen. All these MAbs agglutinate the A 1 , A 1 B and A 2 erythrocytes, while only one of them agglutinates the A 2 B erythrocytes. The agglutination activity of 3 of these MAbs is inhibited by N‐acetyl‐ D ‐galactosamine ( D ‐GalNAc). Dissociation constants of complexes of these MAbs with D ‐GalNAc are approximately 2.2times10 ‐3 mol/1.