Premium
Murine Monoclonal Antibody Suitable for Use as an Rh Reagent, Anti‐e
Author(s) -
Bourel D.,
Lecointre M.,
Genetet N.,
GueguenDuchesne M.,
Genetet B.
Publication year - 1987
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1987.tb02997.x
Subject(s) - monoclonal antibody , reagent , microbiology and biotechnology , chemistry , antibody , antigen , bovine serum albumin , monoclonal , reactivity (psychology) , enzyme , albumin , chromatography , biology , immunology , biochemistry , medicine , pathology , alternative medicine
. The production by the hybridoma technique of a monoclonal antibody which behaves as an anti‐e agglutinin is described. A reagent was made from ascitic fluid diluted in RPMI 1640 culture medium and bovine serum albumin. All red blood cells bearing the e antigen were agglutinated; no reactivity was recorded with untreated EE red cells suspensions. However, cross‐reactivity was observed with enzyme‐pretreated EE cells. The characteristics of this monoclonal antibody are described and are in accordance with the standard references.