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Identification of Anti‐Le a by Platelet Complement Fixation
Author(s) -
Ando Bunei,
Ibayashi Hiroshi
Publication year - 1986
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1986.tb04872.x
Subject(s) - platelet , complement (music) , identification (biology) , medicine , complement fixation test , immunology , antibody , biology , genetics , serology , phenotype , botany , complementation , gene
. Two anti‐Le a sera which were able to detect Le a antigen on platelets were identified in a screening for anti‐platelet antibodies by means of a platelet complement fixation test. These two antisera hemolyzed erythrocytes without enzyme treatment. The anti‐Le a activity could be completely absorbed by red cells, platelets and lymphocytes of Le(a+b‐) donors but not by cells from Le(a‐b+) or Le(a‐b‐) donors. The antibody activity against red cells was eliminated by treatment of the antisera with dithiothreitol, thereby suggesting that the activity resided in the IgM class of immunoglobulins. As the anti‐Le a was more reactive at 37°C than at room temperature against both red cells and platelets, we suggest that transfusion of platelets of Le a ‐negative donors should be considered for patients with this type of anti‐Le a .