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Detection of Anti‐Le a in Le(a‐b+) Individuals by Kinetic ELISA
Author(s) -
Cowles W.,
Spitalnik S. L.,
Blumberg N.
Publication year - 1986
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1986.tb04871.x
Subject(s) - alloimmunity , antibody , autoimmunity , isoantibodies , microbiology and biotechnology , immunology , hemagglutination , biology
. Utilizing a kinetic enzyme‐linked antiglobulin binding assay, eight examples of anti‐Le a were detected in individuals whose Lewis phenotype is Le(a‐b+). These antibodies were not detectable by routine hemagglutination techniques, but were indistinguishable from anti‐Le a made by Le(a‐b‐) individuals in terms of their immunologic fine specificity. However, unlike most anti‐Le a antibodies from Le(a‐b‐) individuals, none of the anti‐Le a antibodies had an IgG component. Anti‐Le a antibodies in Le(a‐b+) individuals may represent immune responses on the continuum between autoimmunity and alloimmunity.