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Two Examples of Low Ionic Strength‐Dependent Autoagglutinins with Anti‐Pr 1 Specificity
Author(s) -
O'Neill Patricia,
Shulman Ira A.,
Simpson Ronald B.,
Halima Diana,
Garratty George
Publication year - 1986
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1986.tb04856.x
Subject(s) - ionic strength , hemolysis , neuraminidase , in vitro , enzyme , chemistry , ionic bonding , sialoglycoproteins , biochemistry , immunology , biology , glycoprotein , ion , organic chemistry , aqueous solution
. Two low ionic strength‐dependent autoagglutinins were studied and found to have anti‐Pr 1 specificity. This specificity was determined by studies with enzyme‐treated and neuraminidase‐treated human red blood cells (RBCs), animal RBCs and chemically‐modified sialoglycoproteins, all suspended in a low ionic strength solution (LISS). Both IgM complement‐binding cold agglutinins had a wide thermal range and caused in vitro hemolysis of some LISS‐suspended RBCs at 37°C. Compatible blood was found for these patients by using techniques that did not employ LISS.