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Fc Receptors for IgG 1 and IgG 3 on Human Mononudear Cells ‐An Evaluation with Known Levels of Erythrocyte‐Bound IgG
Author(s) -
Zupańska Barbara,
Thomson Eileen E.,
Merry Anthony H.
Publication year - 1986
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1986.tb04854.x
Subject(s) - antibody , peripheral blood mononuclear cell , immunology , receptor , immunoglobulin g , fragment crystallizable region , microbiology and biotechnology , rosette (schizont appearance) , chemistry , immunoglobulin fc fragments , rosette formation , biology , in vitro , biochemistry
. The Fc receptors on mononuclear cells were investigated by a rosette technique in which human erythrocytes were sensitized with a known number of molecules of anti‐Rh antibodies (IgG 1 or IgG 3 ). The number of IgG molecules was quantitated by a radiometric antiglobulin test. The present quantitative evaluation reveals that (1) there is a logarithmic relationship between the proportion of rosettes and the amount of erythrocyte‐bound immu‐noglobulin for both types of mononuclear cells, and for both subclasses; (2) similar percentage of rosettes can be obtained with fewer IgG 3 than IgG 1 molecules (about 1:4); (3) for a given number of erythrocyte‐bound immuno‐globulins a higher percentage of rosettes is observed with monocytes than with lymphocytes (ratios of about 3:1 for IgG 1 and 5:1 for IgG 3 ); (4) the minimum number of IgG 3 molecules for adherence is 180–460 for monocytes, 520‐1,300 for lymphocytes, whilst for IgG 1 the numbers are 1,180‐4,300 for monocytes and 3,400‐14,200 for lymphocytes; (5) the minimum levels of sensitization by alloantibodies for adherence should be detectable by the antiglobulin test.