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Room Temperature, Microtray Chromogenic Assay of Factor VIII:C
Author(s) -
Prowse C.,
Hornsey V.,
McKay G.,
Waterston Y.
Publication year - 1986
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1986.tb04840.x
Subject(s) - chromogenic , medicine , chromatography , chemistry
. Commercial reagents of non‐human origin were used to provide a room temperature, microtray chromogenic assay for factor VIII. The assay allows determination of factor VIII in the range 15–150% of normal plasma levels with a coefficient of variation of 3–4%. Haemophilic plasmas and factor VIII concentrates may also be assayed by varying the pre‐dilution step. A good correlation was demonstrated with results obtained using an established one‐stage clotting assay (r = 0.9985).