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Reduced Survival of Isotope‐Labelled Rh(D)‐Negative Donor Red Cells in a Patient with Anti‐LW ab
Author(s) -
Herron R.,
Bell A.,
Poole J.,
Clark M.,
Smith D. S.,
Hamblin T. J.
Publication year - 1986
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1986.tb01975.x
Subject(s) - rh blood group system , medicine , chemistry , isotope , red cell , immunology , antibody , physics , quantum mechanics
. The serum of an 85‐year‐old Caucasian male with no history of blood transfusion contained an IgG3 antibody with anti‐LW ab specificity. The antibody failed to react with dithiothreitol‐treated red cells, and there was a marked reduction in titre of the antibody with pronase‐treated cells, findings consistent with an antibody having this specificity. High association values were obtained in a mononuclear phagocyte assay when LW‐positive red cells, sensitised in vitro with the patient's serum antibody, were incubated with peripheral blood monocytes from the patient. In vivo red cell survival studies demonstrated that 99m Tc‐labelled rhesus‐negative (rr), LW‐positive red cells had 53% survival at 1 h. The IgG subclass of the antibody, mononuclear phagocyte assay results and in vivo survival studies predicted a significant reduction in the posttransfusion survival of therapeutic volumes of rhesus‐negative (rr), LW‐positive red cells.