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Improved Procedure for Platelet Freezing
Author(s) -
Dayian G.,
Harris H. L.,
Vlahides G. D.,
Pert J. H.
Publication year - 1986
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1986.tb01971.x
Subject(s) - platelet , serotonin , epinephrine , thrombin , glycerol , diluent , chemistry , bone marrow , cryopreservation , medicine , biochemistry , biology , receptor , nuclear chemistry , embryo , microbiology and biotechnology
. Platelets frozen in glycerol‐glucose frequently aggregated on thawing or reconstitution. This has been eliminated by using a nonplasma diluent (pH 6.5) and a new freezing bag. The results were improved platelet 14 C‐serotonin uptake and readily demonstrable aggregation to ADP, epinephrine, collagen and thrombin. 14 C‐serotonin uptake was at maximum when platelets were frozen at thrombocrits of up to 15%. Hemostatic effectiveness was demonstrated in a severely thrombocytopenic patient with acute myelogenous leukemia in relapse, who was supported entirely with two autologous frozen platelet transfusions for the 13 days before bone marrow activity resumed.