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Thermal Inactivation of Human Immunodeficiency Virus in Lyophilised Blood Products Evaluated by ID 50 Titrations
Author(s) -
Tersmette Matthys,
Goede Ruud E. Y.,
Over Jan,
Jonge Egge,
Radema Horst,
Lucas Cornells J.,
Huisman Han G.,
Miedema Frank
Publication year - 1986
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1986.tb01962.x
Subject(s) - infectivity , cryoprecipitate , titration , chemistry , virology , virus inactivation , reverse transcriptase , virus , blood product , chromatography , biology , medicine , biochemistry , surgery , polymerase chain reaction , inorganic chemistry , gene , fibrinogen
. Inactivation of human immunodeficiency virus (HIV) in lyophilised small pool cryoprecipitate, factor VIII concentrate, prothrombin complex and C 1 ‐esterase inhibitor concentrate by prolonged heat treatment (72 h, 60° C) was studied. Plasma products, inoculated prior to lyophilisation, had infectious titres ranging from 10 7 to 10 10.5 . Residual infectivity (TCID 50 ) was assessed by multiple titrations on H9 cells in a macro system and subsequent detection of virus replication by determining reverse transcriptase activity. Kinetics of inactivation showed a biphasic pattern: during the first 8 h a variable TCID 50 reduction up to 10 4.3 was observed, followed by an additional loss of 10 1 –10 2.7 during the next 64 h. Heat treatment for 72 h resulted in a mean TCID 50 reduction of 10 5 . It is concluded that prolonged heat treatment may lead to the adequate prevention of HIV transmission by lyophilised plasma products.