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An Autoanalyzer Test for the Quantitation of Platelet‐Associated IgG 1
Author(s) -
Levitan Nathan,
Teno Richard A.,
Szymanski Irma O.
Publication year - 1986
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1986.tb00228.x
Subject(s) - autoanalyzer , platelet , chemistry , chromatography , immunoglobulin g , immunology , antigen , medicine
. A new quantitative antiglobulin consumption (QAC) test for the measurement of platelet‐associated IgG is described. In this test washed platelets are incubated with anti‐IgG at a final dilution of 1:2 million. The unneutralized fraction of anti‐IgG remaining in solution is then measured with an Autoanalyzer and soluble IgG is used for calibration. The dose‐response curves depicting the percent neutralization of anti‐IgG by platelets and by soluble IgG were compared in detail and found to be nearly identical, indicating that platelet‐associated IgG can be accurately quantitated by this method. The mean IgG values were 2,287 molecules/platelet for normal adults and 38,112 molecules/platelet for ITP patients. The Autoanalyzer QAC test is a sensitive and reproducible assay for the quantitation of platelet‐associated IgG.