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Inhibition of Immune Phagocytosis by Human Sera with HLA A, B, C and DR but Not with DQ or EM Type Reactivity
Author(s) -
Neppert Jürgen,
Pohl Elisabeth,
MuellerEckhardt Christian
Publication year - 1986
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1986.tb00227.x
Subject(s) - antibody , cytotoxicity , cytotoxic t cell , phagocytosis , immunology , immune system , antigen , antibody dependent cell mediated cytotoxicity , human leukocyte antigen , monocyte , platelet , biology , in vitro , biochemistry , monoclonal antibody
. 613 sera from pregnant women were investigated for inhibition of immune phagocytosis (IPI) by monocytes exposed to anti‐D (Rh)‐sensitized human red blood cells. IPI was detected in 42 (26%) of 165 and in 78 (17%) of 448 sera assessed against monocytes from 15 or 5 panel donors, respectively. All IPI‐positive sera reacted in an allotypic pattern. Eight IPI‐positive sera tested with autologous monocytes were found to be nonreactive. Upon comparative analysis of 448 sera, a significant correlation was found between the specific patterns of IPI and lymphocytotoxicity by HLA antibodies. In addition, 13 sera (4%) were positive for IPI, but not for lymphocytotoxicity. Twenty IPI‐positive sera were tested by indirect immunofluorescence against platelets and T lymphocytes and revealed IgG antibodies in 16 and 11 sera, respectively. IPI activity could be removed from 8 out of 10 positive sera by platelet pool absorption. While virtually all sera exhibiting HLA, A, B or C‐like activity (cytotoxicity with all cells) or HLA DR‐like activity (exclusive cytotoxicity with B lymphocytes and monocytes) were IPI‐positive, IPI was infrequently observed with sera containing HLA DQ‐like activity (cytotoxicity with B lymphocytes only). IPI was also rarely seen with sera cytotoxic only to monocytes and not at all with sera containing antibodies against endothelial/monocyte antigens. We conclude that IPI is caused by cytotoxic as well as noncytotoxic HLA A, B, C, DR‐specific antibodies. This effect may bear significance for the maternal immune response against fetal antigens and may be useful for pretransplant histocompatibility testing.