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A Microtiter Modification of Granulocyte Immunofluorescence 1
Author(s) -
Press C.,
Kline W. E.,
Clay M. E.,
McCullough J.
Publication year - 1985
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1985.tb00777.x
Subject(s) - granulocyte , immunofluorescence , chromatography , conjugate , antibody , reagent , chemistry , immunology , medicine , mathematics , mathematical analysis
. The granulocyte immunofluorescence test (GIFT) is valuable for detecting allogeneic and autologous granulocyte antibodies. However, the original tube technique (macro GIFT) requires 50–100 μl of serum and 10 6 granulocytes with time‐consuming washing steps which cause cell loss. We report a modification (micro GIFT) using microtiter trays which requires only 20 μl of serum and 2 times 10 5 granulocytes. The modified method conserves reagents, reduces the time required for washing by 67%, decreases cell loss in washing by 74%, reduces the volume of conjugate required by 67%, and compares favorably with the macro GIFT in accuracy, specificity, and sensitivity.