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Inhibition of Human T Lymphocyte Proliferation in vitro by Commercial Factor VIII Concentrates
Author(s) -
Wang Y.,
Beck E.A.,
Furlan M.,
Weck A.L.
Publication year - 1985
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1985.tb00194.x
Subject(s) - phytohaemagglutinin , in vitro , thymidine , mixed lymphocyte reaction , monoclonal antibody , lymphocyte , immunology , chemistry , dialysis , microbiology and biotechnology , stimulation , antibody , medicine , biology , biochemistry , endocrinology , t cell , immune system
. Several investigators recently described a T lymphocyte subset abnormality (reversed helper/suppressor cell ratio) in haemophiliacs, especially following previous sub‐stitution therapy. We studied in vitro the potential effects of two commercial factor VIII concentrates on incorporation of 3 H‐thymidine by isolated human peripheral blood lymphocytes after stimulation with either phytohaemagglutinin or monoclonal antibody OKT 3 , Dose‐related inhibition of thymidine incorporation was found for both concentrates but none using the purified main protein constituents, i.e. factor VIII, fibrinogen or fibronectin. Furthermore, the ‘inhibitor’ was completely removed by dialysis.