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Detection of IgG Anti‐Lewis (a) Antibodies in Cord Sera by Kinetic Elisa
Author(s) -
Spitalnik S.,
Cowles J.,
Cox M. T.,
Blumberg N.
Publication year - 1985
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1985.tb00177.x
Subject(s) - hemolytic disease of the newborn (abo) , antibody , antigen , cord blood , hemagglutination assay , hemagglutination , immunology , fetus , placenta , enzyme , medicine , microbiology and biotechnology , virology , chemistry , pregnancy , biology , biochemistry , titer , genetics
. Lewis blood group antibodies rarely, if ever, cause hemolytic disease of the newborn. This observation has been attributed to the absence both of Lewis antigens on fetal cells and of maternal IgG Lewis antibody. In the present study, sera from 13 mother‐infant pairs were tested for the presence of anti‐Lewis (a) by hemagglutination and by a sensitive and specific kinetic enzyme‐linked immunosorbent assay. By routine hemagglutination methods, anti‐Le a was present in all maternal samples but absent in all cord samples. By kinetic enzyme‐linked immunosorbent assay, IgG anti‐Le a was present in 13 of 13 maternal samples and in 12 of 13 cord samples. These results indicate that IgG anti‐Le a antibodies are common and do cross the placenta. This suggests that they do not cause hemolytic disease of the newborn because of the low levels of Lewis antigens on fetal red cells.