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A Protocol for Sensitive Large‐Scale Screening of Blood Donors for IgA Deficiency
Author(s) -
Hunt A.F.,
Allen D.L.,
Aries D.L.,
Strange J.J.
Publication year - 1985
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1985.tb00151.x
Subject(s) - iga deficiency , immunology , medicine , protocol (science) , immunoglobulin a , antibody , hemagglutination , immunoglobulin g , pathology , alternative medicine
Abstract. A protocol for large‐scale screening of blood donors for IgA deficiency using a tanned cell passive haemagglutination‐inhibition (PHI) technique and enzyme‐linked immunosorbent assay (ELISA) is described. Evidence presented suggests that native serum from a case of IgA paraproteinaemia, suitably diluted and heated to 56°C for 60 min, can act as a satisfactory and simple alternative to purified IgA preparations in the PHI test. When PHI and ELISA techniques were used in combination to screen 41,851 random donors, 58 were found to contain less than 200 ng/ml of IgA and were classified as totally IgA‐deficient (1:721). This is in close agreement with the data from other workers.