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Metabolism of Guanosine in Human Erythrocytes
Author(s) -
Ericson Åke,
Niklasson Frank,
Verdier CarlHenric
Publication year - 1985
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1985.tb00150.x
Subject(s) - guanosine , metabolism , nucleotide , incubation , chemistry , guanine , high performance liquid chromatography , phosphate , chromatography , purine metabolism , biochemistry , extracellular , purine , enzyme , gene
. The metabolism of guanosine in human erythrocytes has been studied in two different experimental systems – direct incubation and dialysis incubation – the latter allowing continuous addition and removal of substances. Intra‐ and extracellular purine compounds were analyzed using high‐performance liquid chromatography (HPLC). At 37°C, a normal pH (7.4) and a favorably high concentration of inorganic phosphate, guanine nucleotides were synthesized at a substance rate of about 0.17 mmol · h ‐1 (calculated per liter erythrocytes) when guanosine was kept at a concentration of 25 μmol · I ‐1 . At a higher guanosine concentration the rate of synthesis increased only moderately. Erythrocytes loaded with guanylates lost these nucleotides at a rate of 0.023 mmol · h ‐1 at a normal phosphate concentration and somewhat slowlier at a higher phosphate concentration. The metabolism kept the guanylates in an equilibrium that was similar to the equilibrium between the adenylates.