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A Mouse Monoclonal Antibody with Anti‐A,(B) Specificity Which Agglutinates A x Cells
Author(s) -
Moore S.,
Chirnside A.,
Micklem L.R.,
McClelland D.B.L.,
James K.
Publication year - 1984
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1984.tb03879.x
Subject(s) - monoclonal antibody , polyclonal antibodies , monoclonal , microbiology and biotechnology , abo blood group system , group a , serology , antibody , group b , chemistry , virology , biology , immunology , medicine
. A hybridoma (ES‐15) was obtained by fusing the NS‐1 cell line with spleen cells from a mouse immunised with soluble blood group A 2 substance. The cloned hybridoma culture supernatant was shown to contain an IgM class antibody which strongly agglutinates group A cells and weakly agglutinates group B cells. The serological specificity of this antibody is described as anti‐A,(B) in this report. The abilities of unconcentrated monoclonal anti‐A,(B), a commercial human polyclonal anti‐A,B (group O serum) and a commercial monoclonal anti‐A reagent to detect 15 examples of A x cells were compared by both slide and tube techniques. Using a slide technique monoclonal anti‐A,(B) agglutinated 14 examples of A x cells, human anti‐A,B 2 examples, while monoclonal anti‐A failed to detect any of the A x cells tested. Similar differences in the reactivity of the three antibodies were observed using a tube technique. Data are also presented which show that a 1:1 (v/v) mixture of monoclonal anti‐A,(B) with a monoclonal anti‐B reagent is an effective replacement for human anti‐A,B in ABO grouping procedures.