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Weak D Antigen Testing Using the Groupamatic System
Author(s) -
Glackin K.C.,
Sherwood W.C.,
Hoppe P.A.,
McIntyre K.
Publication year - 1984
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1984.tb01565.x
Subject(s) - antigen , typing , microbiology and biotechnology , reagent , immunology , red cell , medicine , chemistry , biology , genetics
. Reliable detection of the weak D (D u ) antigen has been a problem for the Groupamatic typing equipment. Using several anti‐D sera of known concentration and D u red cells, dose‐response curves produced by the Groupamatic revealed variation in the ability to detect the D u antigen by commercial anti‐D reagents. Variation in the reactivity of the D u antigen among D u ‐positive donors was noted. Studies were conducted to determine optimal conditions for the Groupamatic to detect the weakest D u cells. When these conditions were applied to the retesting, 28 cell samples previously found falsely negative by a number of blood centers were accurately detected.