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Pasteurization of C1 Inactivator in the Presence of Citrate Salts 1, 2
Author(s) -
Williams Craigenne,
Wickerhauser Milan,
Busby Thomas F.,
Ingham Kenneth C.
Publication year - 1984
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1984.tb00085.x
Subject(s) - chemistry , pasteurization , agarose , immunoelectrophoresis , heparin , chromatography , potassium , antibody , biochemistry , medicine , immunology , organic chemistry
Conditions have been determined under which the C1 inactivator (C1‐INA) can be pasteurized to reduce the risk of transfusion hepatitis associated with its use for replacement therapy in patients with genetic or acquired deficiencies. Recovery of 90% of the biological and immunological activity of a C1‐INA concentrate was achieved following heat treatment for 10 h at 60°C in the presence of 3 M potassium citrate. Crossed immunoelectrophoresis in heparinized agarose was used to demonstrate the ability of the pasteurized C1‐INA to bind heparin and to form a precipitation pattern with antibody which was almost indistinguishable from that of an unheated control. High pressure liquid chromatography and enhancement of the fluorescence of 1,8‐anilinonaphthalene sulfonate were used to show that other proteins present in the concentrate were also stabilized.