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Mouse Monoclonal Antibodies with Anti‐A, Anti‐B and Anti‐A,B Specificities; Some Superior to Human Polyclonal ABO Reagents
Author(s) -
Messeter L.,
Brodin T.,
Chester M. A.,
Löw B.,
Lundblad A.
Publication year - 1984
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1984.tb00074.x
Subject(s) - abo blood group system , polyclonal antibodies , antibody , monoclonal antibody , monoclonal , immunology , antigen , blood grouping , spleen , biology , virology , isoantibodies , microbiology and biotechnology , medicine
A series of fusions using mouse myeloma cells and spleen cells from mice immunized with blood group substances or human red cells was performed with the aim of obtaining ABO antibodies suitable for routine blood grouping. Seven strongly agglutinating antibodies against antigens in the ABO system were obtained from 18 fusions. These antibodies were tested extensively in manual and automated routines, and six of them were found to be as good as or better than commercial polyclonal test sera of human origin.