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Mouse Monoclonal Anti‐HBs and Its Use in the Screening of Donated Blood by Elisa
Author(s) -
Els Maryna C.,
Govender Mathaven,
Marimuthu Thanapal,
Bubb Martin O.,
Conradie Jan D.
Publication year - 1984
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1984.tb00070.x
Subject(s) - hbsag , monoclonal antibody , immunoradiometric assay , antibody , conjugate , microbiology and biotechnology , virology , medicine , monoclonal , whole blood , chemistry , immunology , biology , radioimmunoassay , hepatitis b virus , mathematical analysis , virus , mathematics
A method is described for the production of mouse monoclonal antibodies to HBsAg. 4 clones produced antibodies directed at the a and one at the d determinant of HBsAg. These antibodies were conjugated to horse radish peroxidase and used in an Elisa for the detection of HBsAg in donated blood. Antibody C10 produced conjugate which rendered the Elisa as sensitive as a commercially obtainable immunoradiometric assay with which it was compared. Conditions for this assay were optimised and it may be used as a rapid (1 h) test for detecting HBsAg‐positive blood. It is practicable to read this Elisa by eye instead of photometrically and it can be thus used under field conditions or in emergencies.