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Immunochemical Characterization of a Monoclonal Anti‐Le b Blood Grouping Reagent
Author(s) -
Messeter L.,
Brodin T.,
Chester M.A.,
Karlsson K.A.,
Zopf D.,
Lundblad A.
Publication year - 1984
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1984.tb00054.x
Subject(s) - abo blood group system , monoclonal antibody , glycoconjugate , antibody , microbiology and biotechnology , chemistry , hemagglutination , immunochemistry , monoclonal , biology , biochemistry , immunology
A haemagglutinating monoclonal anti‐Le bL antibody has been obtained from a mouse‐mouse hybridoma after immunization with the Le b ‐active oligosaccharide lacto‐N‐difucohexaose I, coupled to edestin. Of 13 other antibodies obtained, one was able to agglutinate enzyme‐treated erythrocytes of all ABO groups and three more were considered to be anti‐Le bH haemagglutinins. The remaining nine antibodies did not agglutinate red cells. The specificity of the stronger of the anti‐Le bL haemagglutinins was examined by studying its binding to a number of glycoconjugates and oligosaccharides.