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Competition between ABO and Le Gene Specified Enzymes
Author(s) -
Pendu J. Le,
Lemieux R. U.,
Dalix A. M.,
Lambert F.,
Oriol R.
Publication year - 1983
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1983.tb01926.x
Subject(s) - antigen , abo blood group system , enzyme , phenotype , saliva , microbiology and biotechnology , gene , biology , chemistry , genetics , biochemistry
. Radioimmunoassays were prepared using two anti‐A and one anti‐B reagents. The specificity of the procedures was assessed with 13 artificial antigens. The amounts of A and B natural antigens in saliva of ABH secretors of known Lewis phenotype were measured with these assays. The results confirmed that the average amount of A antigen is lower in Lewis‐positive (Le b ) than in Lewis‐negative (Le d ) donors and in A 2 than in A 1 donors. However, the differences among the four combined A and Lewis phenotypes were only supported by significantly lower amounts of A antigenic determinants in A 2 Le b as compared to the other three phenotypes (A 1 Le b , A 1 Le d and A 2 Le d ) that had similar amounts of A antigenic determinants. No Lewis‐related difference could be detected in the amounts of B antigens between BLe b and BLe d donors. The results are discussed in terms of competition between A, B and Lewis‐gene‐specified enzymes for their common acceptors. The difference in the efficiency of the A 2 enzyme as compared to that of the A 1 enzyme is proposed as a possible explanation for the A 1 ‐A 2 phenotypic difference.