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A Detailed Serological Study of Five Anti‐Jk a Sera Reacting by the Antiglobulin Technique
Author(s) -
Howell P.,
Giles C.M.
Publication year - 1983
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1983.tb01897.x
Subject(s) - serology , complement (music) , immunology , antibody , sensitization , medicine , virology , biology , genetics , complementation , gene , phenotype
. Detailed serological investigation of five examples of anti‐Jk a revealed considerable heterogeneity in their characteristics. All the sera were investigated by the antiglobulin technique, with and without complement and by a range of sensitization and testing methods. Three were readily detected in the presence of complement, while two were detectable with anti‐IgG, providing a spin test was used. Complement was bound by all and resulted in enhanced reactions, particularly when slide/tile tests were used. The study emphasizes that anti‐complement (C3) is an essential component of polyspecific antiglobulin reagents used in cross‐matching, enabling the ready detection of these clinically important antibodies.