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Rapid Phenotyping of C3 by Immunofixation on Cellulose Acetate Strips
Author(s) -
Germenis A.,
Babionitakis A.,
Fertakis A.
Publication year - 1982
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1982.tb01117.x
Subject(s) - immunofixation , cellulose acetate , electrophoresis , chromatography , agarose gel electrophoresis , agarose , antiserum , chemistry , cellulose , microbiology and biotechnology , biochemistry , biology , immunology , gene , antibody , monoclonal antibody , monoclonal
Determination of the genetically controlled variants of the C3 system has been accomplished by electrophoresis on cellulose acetate membrane followed by immunofixation with a specific anti‐C3 antiserum. The results have been confirmed with the use of reference sera and compared with those obtained from conventional high voltage agarose gel electrophoresis. The interference of some electrophoretic variables on the technique has been investigated. The method is rapid, accurate, reliable and gives good separation of at least the three common C3 phenotypes.