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Modulation of Complement Lysis of Human Erythrocytes by the Membrane Lipid Viscosity
Author(s) -
Cohen A.M.,
Shinitzky M.
Publication year - 1982
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1982.tb01112.x
Subject(s) - lysis , hemolysis , cholesterol , chemistry , phosphatidylcholine , antiserum , hemolysin , red blood cell , complement system , membrane , biochemistry , biology , immunology , antibody , phospholipid , virulence , gene
Human red blood cells (H‐RBC), sensitized by specific rabbit antihuman hemolysin, showed a significant increase in susceptibility to complement lysis after rigidification of the membrane lipid layer by incorporation of cholesterol (p < 0.001). Conversely, fluidization of the membrane lipid layer by treatment with phosphatidylcholine for cholesterol depletion displayed increased tolerance to the complement lysis (p < 0.01). Antisera, obtained from 6 rabbits immunized with untreated H‐RBC, were tested separately. The average serum concentration, exerting 50% hemolysis, was 1.1 ± 0.4% for cholesterolenriched H‐RBC and 16.3 ± 7.5% for cholesterol‐depleted H‐RBC.