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Production of Direct Agglutinins by the Combination of Incomplete Antibodies to Protein A‐Bearing Staphylococcus aureus Cells
Author(s) -
Deary I.J.,
Moore S.,
Pepper D.S.
Publication year - 1982
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1982.tb01101.x
Subject(s) - staphylococcus aureus , antibody , saline , in vitro , protein a , microbiology and biotechnology , titer , chemistry , immunology , biology , bacteria , biochemistry , genetics , endocrinology
A novel method is described for the production of saline‐reacting antibodies from ‘incomplete’ blood grouping sera. IgG anti‐D is combined to protein A‐bearing Staphylococcus aureus (SA), forming a polyvalent anti‐D assembly. The SA‐anti‐D has a high titre in saline and is stable on 4°C storage and lyophilisation. The material was successful in Rh(D) typing cells sensitised in vitro with various antibodies. Saline‐reacting SA‐anti‐Kell and SA‐anti‐c̄ were also produced.