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Posttransfusion Viability of Rabbit Erythrocytes Preserved in a Medium Containing Inosine, Adenine, and Isoosmotic Sucrose
Author(s) -
Nakuo Toshiko,
Nugai Fumiko,
Nakao Mukoto
Publication year - 1982
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1982.tb01098.x
Subject(s) - sucrose , inosine , chemistry , adenosine triphosphate , biochemistry , adenosine
. A sucrose medium containing relatively great amounts of adenine and inosine (AIS‐CPD) was more effective for maintaining adenosine triphosphate (ATP) and 2,3‐diphosphoglyceric acid (DPG) levels of human erythrocytes for more than 35 days at 4°C than control ACD‐packed cells, sucrose medium (S‐CPD), and sucrose medium containing a small amount of adenine. Then rabbit erythrocytes were used prior to in vivo test of human cells. (1) After storage for 5 weeks the ATP level (μmol/g Hb) was 3.25 in AIS‐CPD, 2.65 in AISNa‐CPD (AIS‐CPD containing NaCl plus sucrose instead of sucrose), 0.77 in S‐CPD, and 0.42 in CPD (fresh: 5.39; n = 4–10). (2) The DPG level (μmol/g Hb) was AIS‐CPD = fresh > AISNa‐CPD > S‐CPD ė CPD. (3) The mean corpuscular volume decreased gradually in AIS‐CPD. (4) The fragility of red cells decreased in AIS‐CPD. (5) The posttransfusion viability measured by the 51 Cr method after storage of erythrocytes for 5 weeks was 84.5% in AIS‐CPD, 70.6% in AISNa‐CPD, 59.5% in S‐CPD, and 28.5% in CPD, 24 h after transfusion 14.9 in AIS‐CPD. 9.9 in AISNa‐CPD, 12.8 in S‐CPD, and 8.4 in CPD (fresh: 14.0; n = 7–8).