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Improved Assay for Detection of Platelet‐Specific Pl A1 Antibodies in Neonatal Alloimmune Thrombocytopenia
Author(s) -
MuellerEckhardt C.,
Kayser W.,
Förster Christa,
MuellerEckhardt Gertrud,
Ringenberg C.
Publication year - 1982
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1982.tb00529.x
Subject(s) - neonatal alloimmune thrombocytopenia , antibody , platelet , medicine , immunology , specific antibody , isoantibodies , pregnancy , biology , fetus , genetics
. A technique is described which permits the detection of platelet‐specific, noncomplement‐fixing (‘blocking’) P1 A1 antibodies in serologically negative sera of mothers from children suffering from neonatal alloimmune thrombocytopenia. The technique consists of (1) antibody enrichment in eluates prepared from maternal serum and P1 A1 ‐positive platelets, and (2) quantitation of antibodies in eluates by the platelet radioactive anti‐IgG test. With this assay, P1 A1 antibodies were demonstrated in 6 out of 7 maternal sera, and anti‐A antibodies in one. It proved valuable also for longitudinal studies for periods up to 30 months after delivery.