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Elution of Group‐Specific Substance A from RBC of Various Subgroups of A and Its Effect on the Agglutination of A x RBC
Author(s) -
Dodd B.E.,
Wood N.J.
Publication year - 1982
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1982.tb00020.x
Subject(s) - elution , agglutination (biology) , titer , chemistry , antigen , antibody , abo blood group system , chromatography , hemagglutination tests , blood group antigens , immunology , medicine , hemagglutination
Experiments are described which demonstrate that when the Landsteiner heat elution technique is used to prepare eluates from group A cells, some A substance as well as anti‐A is released into the eluates. The concentration found is greatest from A 1 RBC and least from A x , suggesting a relationship with the number of antigen sites on RBC of the various subgroups. Further experiments have shown that the presence of this A‐specific substance in eluates accounts for the well‐know phenomenon that anti‐A from selected group O sera, when eluted from A 1 RBC, will not agglutinate A x even though the original serum showed an appreciable titre against A x . The presence of the eluted antigen also suggests an explanation for the behaviour of antibody recovered from the red cells of various subgroups of A towards A x .