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Purification of Anti‐Le c Antibodies with Specificity for β D Gal(1→3)β D GlcNAcO‐ Using a Synthetic Immunoadsorbent
Author(s) -
Pendu J. Le,
Lemieux R.U.,
Oriol R.
Publication year - 1982
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1982.tb00011.x
Subject(s) - antigen , antibody , chemistry , population , oligosaccharide , biology , microbiology and biotechnology , biochemistry , immunology , medicine , environmental health
Artificial antigens: Antibodies were raised in rabbits with β D Gal(13) β D GlcNAc‐BSA and purified by absorption elution on a column of the synthetic oligosaccharide covalently bound to a silicate instead of BSA. The purified antibodies agglutinated specifically erythrocytes from Le (a‐b‐) nonsecretor donors (Le c ). Natural antigens: Antibodies were raised in goats using boiled saliva from O/O, le/le, se/se donors. The population of antibodies which was adsorbed on a column consisting of β D Gal(1→3) β D GlcNAc‐O‐R (type 1 chain precursor disaccharide) groupings attached to a silicate was collected and then refined by passage through a column which consisted of similarly immobilized α L Fuc(1→2)β D Gal(1→3)β D GlcNAc‐(H type 1), β D Gal(1→3)‐[α L Fuc(1→4)]β D GlcNAc‐(Le a ), and β D Gal(1→4)β D GlcNAc‐ (type 2 core chain precursor) groupings. The refined antibodies proved to have a reaction pattern with salivas that is the same as that previously reported for anti‐Le c sera. The data obtained with natural and artificial antigens are thus compatible with the biosynthetic pathway for the formation of the Lewis antigenic determinants proposed by Graham and suggest that the Le c antigen may simply be the type 1 precursor chain which is required for the formation of the Le a , Le d and Le b antigenic determinants.