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Differential Coating of Human Red Blood Cells with C4 or C3 in a Low Ionic Strength Medium
Author(s) -
Heier H. E.,
Kornstad L.,
Nordhagen R.
Publication year - 1981
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1981.tb01045.x
Subject(s) - incubation , ionic strength , coating , chemistry , chromatography , incubation period , ionic bonding , blood preservation , nuclear chemistry , ion , biochemistry , andrology , medicine , aqueous solution , organic chemistry
Abstract An EDTA‐containing, low ionic strength medium, pH 5.1, can be used for incubation of blood to obtain coating of red blood cells with C4 or C3. The coating achieved depends on incubation temperature (4 or 37°C) and on whether or not CaCl 2 or MgCl 2 is added to the medium. The optimal concentration of EDTA appears to be 2 m M under the present conditions. This is equimolar to the final concentration of the added CaCl 2 /MgCl 2 . The choice between CaCl 2 and MgCl 2 depends on whether blood without anticoagulant or ACD blood is employed. Coated cells keep satisfactorily for 5 weeks at 4°C in Alsever's solution as well as after freezing and thawing. C3‐coated cells can be converted to C3dcoated ones by incubation in normal, compatible, EDTA‐containing serum at 37°C.