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Protease Modification Enhances Anti‐D Binding to Nucleated Red Blood Cell Precursors 1
Author(s) -
Rearden A.,
Masouredis S.P.
Publication year - 1981
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1981.tb01030.x
Subject(s) - protease , red blood cell , erythropoiesis , antigen , blood cell , chemistry , membrane , cell membrane , biochemistry , microbiology and biotechnology , biology , enzyme , immunology , medicine , anemia
Protease modification of nucleated red blood cell precursors resulted in significant enhancement of anti‐D binding, indicating the presence of cryptic Rh determinants within the plasma membrane of the developing erythroid cell. Protease‐modified nucleated precursor cells bound less anti‐D than protease‐modified mature red blood cells, however, suggesting that the total content of D antigen in the developing erythropoietic plasma membrane is reduced. These findings support the hypothesis that the D antigen, an integral membrane protein, is progressively synthesized during erythropoiesis.