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Red Cell ATP and 2,3‐Diphosphoglycerate Concentrations as a Function of Dihydroxyacetone Supplementation of CPD Adenine
Author(s) -
Moore G.L.,
Ledford M.E.,
Brummell M.R.
Publication year - 1981
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1981.tb01005.x
Subject(s) - diphosphoglycerate , dihydroxyacetone , chemistry , medicine , red blood cell , endocrinology , zoology , red cell , biochemistry , whole blood , biology , hemoglobin , glycerol
. Units of CPDA‐1 whole blood were subdivided and each treated with additions of dihydroxyacetone (DHA) to give final concentrations from 0 to 80m M . The ‘optimum’ concentration of DHA to maintain 2,3‐diphosphoglycerate (2,3‐DPG) with minimal loss of ATP during 42 days of storage appeared to be 30m M of DHA. With this formulation, red cell 2, 3‐DPG concentrations rose to 130–140% of normal by 14 days and then decreased in a near‐linear manner to 50–60% normal by 42 days, while maintaining adequate ATP levels. In addition, packed red cells were prepared from CPD fresh blood and treated with adenine, glucose, and various concentrations (0‐80m M of DHA. The cells also responded most favorably to 30m M DHA, although the response was not as positive as whole blood. This concentration of DHA produced nearly 100% maintenance of 2,3‐DPG at 14 days with subsequent fall to 30% of normal by 42 days.