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A New Preparation of Modified Immune Serum Globulin (Human) Suitable for Intravenous Administration
Author(s) -
Schroeder Duane D.,
Tankersly Donald L.,
Lundblad John L.
Publication year - 1981
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1981.tb00725.x
Subject(s) - dithiothreitol , iodoacetamide , disulfide bond , chemistry , alkylation , globulin , chromatography , cleavage (geology) , cysteine , biochemistry , medicine , immunology , catalysis , biology , enzyme , paleontology , fracture (geology)
. Immune serum globulin (ISG) prepared by Cohn cold alcohol fractionation of pooled human plasma was reduced with dithiothreitol (DTT) and alkylated with iodoace‐tamide and other alkylating agents. Our results show that there are a few labile inter heavy chain disulfide bonds in ISG which react rapidly under mild, non dissociating conditions. The extent of disulfide cleavage is controlled primarily by the ratio of DTT to ISG until about 4–5 disulfide bonds have been reduced. We report detailed studies on the variables of ISG concentration, DTT to ISG ratio, pH, and time, leading to a chemically modified ISG that has a controlled and limited number of reduced and alkylated disulfide bonds.