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Elution of Blood Group Antibodies from Red Cells
Author(s) -
Oss C.J.,
Beckers D.,
Engelfriet C.P.,
Absolom D.R.,
Neumann A. W.
Publication year - 1981
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1981.tb00722.x
Subject(s) - elution , bovine serum albumin , chromatography , chemistry , ultrafiltration (renal) , dimethyl sulfoxide , albumin , antibody , dialysis , phosphate buffered saline , aqueous solution , saline , red blood cell , biochemistry , immunology , medicine , surgery , organic chemistry
. By slowly lowering the surface tension of the aqueous medium through the admixture of 47.5% dimethyl sulfoxide (DMSO) and 0.1% bovine serum albumin (BSA) and by raising the pH to 9, complete elution of A, D, and K antibodies from sensitized erythrocytes (RBC) could be achieved. The eluates comprising the antibodies were subjected to dialysis to remove the DMSO and to neutralize the pH, and to ultrafiltration to remove the excess water. Recovery of a sizeable proportion of the eluted RBC (of blood groups A and K) proved possible by slow and careful removal of the DMSO with phosphate‐buffered saline containing 2% BSA.