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In vitro Interactions of 51 r in Human Red Blood Cells and Hemolysates 1, 2
Author(s) -
Kuehl Gary V.,
Harkness Donald R.,
Skrabut Eugene M.,
Bechthold Duane A.,
Emerson Charles P.,
Valeri C. Robert
Publication year - 1981
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1981.tb00704.x
Subject(s) - hemoglobin , intracellular , sodium citrate , chemistry , in vitro , red blood cell , biochemistry , microbiology and biotechnology , biology , medicine , pathology
. The intracellular distribution of radioactivity was studied in normal and sickle erythrocytes labeled with sodium 51 Cr chromate. Both types of cells had a higher fraction of 51 Cr bound to hemoglobin when labeled in the presence of ACD at a pH of 7.09 than when labeled in the presence of CPD at a pH of 5.96. Citrate at a pH of 5.96 or less entered the red blood cells and decreased the 51 Cr binding hemoglobin. Binding of 51 Cr to hemoglobin within the red cells was also reduced when the ATP and DPG levels in the red blood cells were elevated. Studies with hemoglobin solution showed that 51 Cr binding to hemoglobin was influenced by 2,3‐DPG, ATP and citrate.