Premium
Red‐Cell Enzyme Activities and Properties of Mutant Pyruvate Kinase after 1‐Year Storage in a ‐80°C Freezer
Author(s) -
Ishida Yoji,
Miwa Shiro,
Takegawa Susumu,
Fujinami Noboru,
Yamato Kunio
Publication year - 1981
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1981.tb00674.x
Subject(s) - pyruvate kinase , red cell , mutant , phosphoglycerate kinase , enzyme , biochemistry , triosephosphate isomerase , pyruvate kinase deficiency , kinase , red blood cell , chemistry , microbiology and biotechnology , biology , medicine , glycolysis , gene
. Red cells from 5 healthy adults and an unstable mutant pyruvate kinase (PK), PK Maebashi, were stored for 12 months in a ‐80°C freezer. With an exception of triosephosphate isomerase and phosphoglycerate kinase activities, normal red‐cell enzyme activities remained essentially unchanged. Mutant PK was characterized by the methods recommended by the International Committee for Standardization in Haematology (ICSH). Normal control and PK Maebashi remained essentially unchanged in their characteristics after storage in a ‐80°C freezer for 12 months. These results indicate that the red cells stored over a long term in a ‐80°C freezer can be used for the study of unstable mutant PK.