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Selection of Plasma for Hepatitis B Immune Globulin Using a Low Cost Radioimmunoassay
Author(s) -
Hopkins R.,
Ross S.,
Barr A.,
McClelland D. B. L.
Publication year - 1981
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1981.tb00662.x
Subject(s) - hbsag , polyethylene glycol , radioimmunoassay , antibody , globulin , hepatitis b , chromatography , chemistry , hepatitis b virus , immunology , microbiology and biotechnology , medicine , biology , virus , biochemistry
. Polystyrene beads coated with pasteurised polyethylene glycol‐precipitated HBsAg were used to detect anti‐HBs by a solid phase inhibition assay employing unreacted 125 I‐anti‐HBs which had previously been ‘processed’ by polyethylene glycol precipitation and used to test for HBsAg. A weak anti‐HBs (approximately 0.1 IU/ml) positive control was used to establish a target level of reactivity. 16,035 freshly donated units of blood, a 90‐sample coded anti‐HBs panel and a selection of hyperimmune globulins were investigated. All positive results were confirmed by a standard commercial assay. Antibody content of selected samples was quantitated in international units. A target level of anti‐HBs activity is suggested for donor plasma to be fractionated for the production of hepatitis B immune globulin.