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Demonstration of β 2 ‐Microglobulin in the Kidney by Direct Immunofluorescent Staining
Author(s) -
ChungPark Moonja,
Enojo Paz Villamil,
Hall Philip W.
Publication year - 1980
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1980.tb04505.x
Subject(s) - beta 2 microglobulin , staining , pathology , kidney , beta (programming language) , fluorescein isothiocyanate , basement membrane , kidney disease , cytoplasm , immunofluorescence , kidney tubules , chemistry , medicine , microbiology and biotechnology , biology , antibody , immunology , fluorescence , biochemistry , physics , quantum mechanics , computer science , programming language
. Direct immunofluorescent staining with fluorescein isothiocyanate conjugated goat anti‐human β 2 ‐microglobulin (β 2 M) was used to determine the localization of β 2 M in kidney tissue, obtained by biopsies or nephrectomies for various renal diseases. β 2 M was found in the renal tubular epithelial cell cytoplasm and occasionally in the tubular basement membranes in 48 out of 79 pathologic specimens tested, but not in 3 normals. β 2 M was seen in the tubular cells in all cases of primary tubulointerstitial disease and in all cases with secondary tubular damage associated with glomerular disease or systemic disease. This supports the concept that β 2 M is metabolized in proximal tubular epithelial cells, and its deposition indicates impairment of tubular handling.