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Blood Group ABH Antigens on Human Cord Red Cells
Author(s) -
SchenkelBrunner Helmut
Publication year - 1980
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1980.tb04497.x
Subject(s) - fractionation , abo blood group system , cord blood , carbohydrate , chemistry , incubation , glycoprotein , antigen , transferase , cell , blood group antigens , red cell , red blood cell , biochemistry , microbiology and biotechnology , biology , immunology , enzyme , medicine , chromatography
. When the blood group H sites of cord erythrocytes obtained from newborn infants of groups O, A, B and AB were labelled specifically by incubation of the whole cells with the A 1 gene dependent a‐N‐acetylgalactosaminyl transferase in the presence of UDP‐N‐acetyl [ 14 C]‐galactosamine, the incorporation of radioactivity was considerably lower than that found for cells from adults. Based on the amount of label recovered in the membranes, average values of 326,000 H sites per single O cell and 68,000 H sites per single A, B and AB cell were calculated. Following fractionation of the stromal blood group substances thus labelled, it was found that, on the average, 66% of the radioactivity was bound to glycoprotein material, 2.7% to glycosphingolipids with short carbohydrate chains, and about 24% to polyglycosylceramides. As these values are similar to those previously determined for O cells from adults, this result shows that there are probably no substantial differences between erythrocytes from adults and newborn infants concerning the overall membrane disposition, but rather provides evidence for variations in the carbohydrate chains of the stromal glyco‐conjugates.