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H 2 O 2 Release from Filtration Leukapheresis‐Procured Leukocytes
Author(s) -
Suzuki Hitoshi,
Kakinuma Katsuko,
Shimizu Masaru,
Sakamoto Hisaharu,
Shimada Kokichi
Publication year - 1980
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1980.tb01868.x
Subject(s) - leukapheresis , phagocytosis , myeloperoxidase , filtration (mathematics) , granulocyte , chemistry , apheresis , dextran , immunology , microbiology and biotechnology , platelet , andrology , chromatography , medicine , biology , inflammation , stem cell , statistics , mathematics , cd34
. Filtration leukapheresis‐procured leukocytes (FL‐leukocytes), which were collected by the elution of filtration columns with vigorous tapping, released a certain amount of H 2 O 2 , even in the absence of any phagocytic stimuli. Furthermore, FL‐leukocytes, eluted with either gentle or vigorous tapping, exhibited a marked release of H 2 O 2 during phagocytosis. The myeloperoxidase (MPO) activity of FL‐leukocytes was lower than that of leukocytes collected by the dextran sedimentation method (DS‐leukocytes). The data suggest that the release of both H 2 O 2 and MPO from FL‐leukocytes may be related to adverse transfusion reactions and abnormal post‐transfusion kinetics of FL‐leukocytes due to their toxic effects on living cells.