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The Applicability of Sodium Dodecylsulfate‐Polyacrylamide Gel Electrophoresis for the Quantitation of Fragments in Immune Serum Globulin Preparations
Author(s) -
Bertolini Maurice J.,
Stryker Martin H.,
Horowitz Marilyn S.
Publication year - 1980
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1980.tb01867.x
Subject(s) - chromatography , chemistry , gel permeation chromatography , polyacrylamide gel electrophoresis , globulin , ultracentrifuge , gel electrophoresis of proteins , gel electrophoresis , sodium dodecyl sulfate , sodium , biochemistry , biology , immunology , organic chemistry , enzyme , polymer
. A comparison of sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE), ultracentrifugation and gel permeation chromatography was investigated for quantitation of fragments in preparations of immune serum globulin. SDS‐PAGE was shown to be the method of choice by virtue of its sensitivity and its ability to clearly separate the products of fragmentation. Gel permeation chromatography and ultracentrifugation yielded similar results but did not approach the sensitivity of SDS‐PAGE.