Premium
Immunochemical Characteristics of Human Anti‐T Antibodies
Author(s) -
Kim Yung D.
Publication year - 1980
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1980.tb01852.x
Subject(s) - radioimmunoassay , hemagglutination , antibody , hemagglutination assay , polyethylene glycol , chemistry , virology , immunology , biology , biochemistry , titer
A specific and sensitive radioimmunoassay was developed to investigate the immunochemical properties of serum anti‐T antibodies. Evidence was presented that the radioimmunoassay can measure non‐agglutinating as well as agglutinating anti‐T antibodies, while the hemagglutination method can detect only the latter type. Although the hemagglutination method failed to detect anti‐T agglutinins in cord sera, the radioimmunoassay results demonstrated the presence of non‐agglutinating antibodies. Presence of 1% polyethylene glycol potentiated some of the originally nonagglutinating antibodies to produce a hemagglutination reaction. Immunochemical characteristics associated with the human anti‐T antibodies are discussed.