Premium
Monoclonal Anti‐A from a Hybrid‐Myeloma: Evaluation as a Blood Grouping Reagent
Author(s) -
Voak D.,
Sacks S.,
Alderson T.,
Takei F.,
Lennox E.,
Jarvis J.,
Milstein C.,
Darnborough J.
Publication year - 1980
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1980.tb01848.x
Subject(s) - monoclonal antibody , reagent , abo blood group system , spleen , antibody , blood grouping , monoclonal , cell culture , chemistry , microbiology and biotechnology , immunology , biology , genetics
A monoclonal anti‐A antibody has been evaluated and found suitable for use as a potent routine ABO grouping reagent, without the use of additives. The IgM anti‐A (MH2/6D4) is secreted into the tissue culture supernatant by a permanent line of cloned cells derived by fusion of anti‐A producing spleen cells and a mouse myeloma cell line. This is a costeffective reagent which should reduce production costs by over 50%. This reagent has the advantages inherent in monoclonal antibodies among them the availability of unlimited quantities of unvarying antibody of known properties.